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antigenic peptide  (Thermo Fisher)


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    Structured Review

    Thermo Fisher antigenic peptide
    Antigenic Peptide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antigenic+peptide/C+PEPTIDE+HUMAN/bio_rxiv__2025__11__05__686769-241-10-55
    Average 95 stars, based on 1 article reviews
    antigenic peptide - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Purification:

    Article Title: The Cdk5 Activator P39 Specifically Links Muskelin to Myosin II and Regulates Stress Fiber Formation and Actin Organization in Lens
    Article Snippet: .. The IgG fraction was purified from the resulting antisera using protein A-agarose beads (Pierce Biotechnology, Inc., Rockford, IL), and the p39 antibody was further purified by affinity chromatography against the antigenic peptide covalently coupled to agarose beads as a secondary amine using the AminoLinkTM kit and AminoLink coupling gel (Pierce). .. Commercially available antibodies for p39 (3275S) and muskelin (ab56135-100) were purchased from Cell Signaling Technology Inc., Danvers, MA and Abcam Inc., Cambridge, MA respectively.

    Affinity Chromatography:

    Article Title: The Cdk5 Activator P39 Specifically Links Muskelin to Myosin II and Regulates Stress Fiber Formation and Actin Organization in Lens
    Article Snippet: .. The IgG fraction was purified from the resulting antisera using protein A-agarose beads (Pierce Biotechnology, Inc., Rockford, IL), and the p39 antibody was further purified by affinity chromatography against the antigenic peptide covalently coupled to agarose beads as a secondary amine using the AminoLinkTM kit and AminoLink coupling gel (Pierce). .. Commercially available antibodies for p39 (3275S) and muskelin (ab56135-100) were purchased from Cell Signaling Technology Inc., Danvers, MA and Abcam Inc., Cambridge, MA respectively.

    Article Title: Transporter regulator RS1 (RSC1A1) coats the trans-Golgi network and migrates into the nucleus.
    Article Snippet: Kroiss, Matthias, Marina Leyerer, Valentin Gorboulev, Thomas Kühlkamp, Helmut Kipp, and Hermann Koepsell.. Transporter regulator RS1 (RSC1A1) coats the trans-Golgi network and migrates into the nucleus.. Am J Physiol Renal Physiol 291: F1201–F1212, 2006.

    Incubation:

    Article Title: CDK12 is a transcription elongation-associated CTD kinase, the metazoan ortholog of yeast Ctk1
    Article Snippet: .. Elution 1 was carried out by a 1-h incubation with end-over-end rocking at 4°C with 1 mM antigenic peptide (ABR/Thermo Scientific) in 100 μL of 100 mM KCl/HGEDP. ..

    Article Title: KIT receptor-positive cells in the bovine corpus luteum are primarily theca-derived small luteal cells
    Article Snippet: The primary antiserum was diluted (concentration 100 mg/ml) in PBS with 0.25% BSA (A-4378, Sigma–Aldrich) and applied at 4 8C overnight. .. To control the specificity of KIT staining, the antiserum was incubated with 100! molar excess of the antigenic peptide (PP-1518, LAB VISION Corp., Fremont, CA, USA) at 4 8C overnight, the immunoprecipitates were removed by repeated centrifugation, and the supernatants applied to sections. .. Next day, slides were successively washed 3! in TBS/Tween and TBS alone, and then incubated with DakoCytomation Envision System (Labelled Polymer-HRP Anti-rabbit, K4002; DakoCytomation) at RT for 30 min.

    Recombinant:

    Article Title: Transporter regulator RS1 (RSC1A1) coats the trans-Golgi network and migrates into the nucleus.
    Article Snippet: Kroiss, Matthias, Marina Leyerer, Valentin Gorboulev, Thomas Kühlkamp, Helmut Kipp, and Hermann Koepsell.. Transporter regulator RS1 (RSC1A1) coats the trans-Golgi network and migrates into the nucleus.. Am J Physiol Renal Physiol 291: F1201–F1212, 2006.

    Centrifugation:

    Article Title: Stenocytosis : a mechanism for supramolecular attack particle transfer at the CTL lytic synapse
    Article Snippet: CTLs were fixed with 3% paraformaldehyde, washed with PBS/3% BSA/HEPES and directly acquired with a Yokogawa CSU-W1 Spinning Disk equipped of a Live Super-Resolution module, a ×100 Plan Apochromat LBDA objective (1.45 oil) (Nikon Instruments) and a Prime 95B Scientific CMOS Camera (Teledyne Photometrics). .. Target cells were either non-pulsed or pulsed with 10 μM antigenic peptide (CMV p65) for 2 h at 37°C and 5% CO 2 in RPMI 5% FCS/HEPES, washed three times, and conjugated, using 1 minute centrifugation at 455g, with antigen-specific human CTLs previously loaded overnight with Wheat Germ Agglutinin (WGA) Alexa Fluor 647 (1 μg/mL) (Molecular Probes, Invitrogen). ..

    Article Title: KIT receptor-positive cells in the bovine corpus luteum are primarily theca-derived small luteal cells
    Article Snippet: The primary antiserum was diluted (concentration 100 mg/ml) in PBS with 0.25% BSA (A-4378, Sigma–Aldrich) and applied at 4 8C overnight. .. To control the specificity of KIT staining, the antiserum was incubated with 100! molar excess of the antigenic peptide (PP-1518, LAB VISION Corp., Fremont, CA, USA) at 4 8C overnight, the immunoprecipitates were removed by repeated centrifugation, and the supernatants applied to sections. .. Next day, slides were successively washed 3! in TBS/Tween and TBS alone, and then incubated with DakoCytomation Envision System (Labelled Polymer-HRP Anti-rabbit, K4002; DakoCytomation) at RT for 30 min.

    Whole Genome Amplification:

    Article Title: Stenocytosis : a mechanism for supramolecular attack particle transfer at the CTL lytic synapse
    Article Snippet: CTLs were fixed with 3% paraformaldehyde, washed with PBS/3% BSA/HEPES and directly acquired with a Yokogawa CSU-W1 Spinning Disk equipped of a Live Super-Resolution module, a ×100 Plan Apochromat LBDA objective (1.45 oil) (Nikon Instruments) and a Prime 95B Scientific CMOS Camera (Teledyne Photometrics). .. Target cells were either non-pulsed or pulsed with 10 μM antigenic peptide (CMV p65) for 2 h at 37°C and 5% CO 2 in RPMI 5% FCS/HEPES, washed three times, and conjugated, using 1 minute centrifugation at 455g, with antigen-specific human CTLs previously loaded overnight with Wheat Germ Agglutinin (WGA) Alexa Fluor 647 (1 μg/mL) (Molecular Probes, Invitrogen). ..

    Control:

    Article Title: KIT receptor-positive cells in the bovine corpus luteum are primarily theca-derived small luteal cells
    Article Snippet: The primary antiserum was diluted (concentration 100 mg/ml) in PBS with 0.25% BSA (A-4378, Sigma–Aldrich) and applied at 4 8C overnight. .. To control the specificity of KIT staining, the antiserum was incubated with 100! molar excess of the antigenic peptide (PP-1518, LAB VISION Corp., Fremont, CA, USA) at 4 8C overnight, the immunoprecipitates were removed by repeated centrifugation, and the supernatants applied to sections. .. Next day, slides were successively washed 3! in TBS/Tween and TBS alone, and then incubated with DakoCytomation Envision System (Labelled Polymer-HRP Anti-rabbit, K4002; DakoCytomation) at RT for 30 min.

    Staining:

    Article Title: KIT receptor-positive cells in the bovine corpus luteum are primarily theca-derived small luteal cells
    Article Snippet: The primary antiserum was diluted (concentration 100 mg/ml) in PBS with 0.25% BSA (A-4378, Sigma–Aldrich) and applied at 4 8C overnight. .. To control the specificity of KIT staining, the antiserum was incubated with 100! molar excess of the antigenic peptide (PP-1518, LAB VISION Corp., Fremont, CA, USA) at 4 8C overnight, the immunoprecipitates were removed by repeated centrifugation, and the supernatants applied to sections. .. Next day, slides were successively washed 3! in TBS/Tween and TBS alone, and then incubated with DakoCytomation Envision System (Labelled Polymer-HRP Anti-rabbit, K4002; DakoCytomation) at RT for 30 min.



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